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Aponi Devoted Fan

Joined: 26 Apr 2007 Posts: 545 Location: New York
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Posted: Wed Jul 11, 2007 8:56 pm Post subject: |
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Ew. I just killed a skeeter on my comp screen. Nasty.
And I WANT to print this out but i'm out of ink, maybe someone should print Marla's reference point version, it might be easier to read that way. _________________ The names Julie.
Bree and Jonas Forever! <3
Grand Master of Praise of the SSJF but Shhh about it 'k? XD
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krmurr87 Devoted Fan
Joined: 13 Nov 2006 Posts: 917
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Posted: Wed Jul 11, 2007 8:56 pm Post subject: |
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Maybe it's somebodies name? Or references from a book? |
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marlasinger Devoted Fan

Joined: 27 Feb 2007 Posts: 736 Location: The 8th Dimension
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Posted: Wed Jul 11, 2007 8:56 pm Post subject: |
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from some random sites:
" One way to repair mRNA is to use ribozymes, RNA molecules that act as 'molecular scissors' by catalysing the cleavage of mRNA at sequence-specific sites."
"Targeting a mutation discovered in an affected dog, they engineered a ribozyme that splices off the mutation-containing 3? section of the mRNA and replaces it with the wild-type sequence. This technique is called ribozyme-mediated trans-splicing."
http://www.vetscite.org/publish/items/001060/index.html
knowing the function and theories of ribozymes will make filling in those blanks a LOT easier, guys. _________________ my love tastes a lot like tofu.
The Bree Solution.
http://one.revver.com/watch/330795
"Also... im in ur canons... supporting all ur marlas."
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tazman2087 Casual Observer
Joined: 29 May 2007 Posts: 45 Location: Alabama
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Posted: Wed Jul 11, 2007 8:58 pm Post subject: |
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Im pretty sure its "Bree Trait Reference Points" _________________ Meh. |
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ohpete Casual Observer

Joined: 11 Jul 2007 Posts: 40
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Posted: Wed Jul 11, 2007 8:59 pm Post subject: |
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Looks like we're this far:
The Bree Solution (that sounds REALLY important )
Objectives:
How to transform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE
Given Facts:
1) Bree's blood contains a special ribozyme mutated and unique only to her.
2) Genome for said ribozyme has a regular hyper mutation that changes with every generation.
3) A non-functioning mutated(?) ribozyme exists and manifests differently in every Hymn of One girl.
4) This portion still functions but you cannot block it because the complimentary strands of RNA won't combine.
Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to ____
2) Create complimentary strand of RNA.
3) Q.E.D. strand will bind to ribozyme and occlude its activity/energy/effeciency(?).
Practical Solution
1) Obtain blood sample of Bree .
2) Synthesize complimentary strand of RNA
3) Inject Bree with this compliementary strand.
4) Q.E.D. ribozymes blocked will be rendered defunctual.
Intended Results
1) Once synthesized and injected into Bree she'll become trait negative
2) Additional consequences (if any) - TBD
______ Reference Points
The steps concerning ribozyme function
RNAse P related -tRNA cleavage
Splicosome related - intron/exon splicing
______related - telomere synthesis
GlmS ribozyme related - fructose 6-phosphate pathway |
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kellylen The Order of Denderah

Joined: 21 Nov 2006 Posts: 2823 Location: New Jersey
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Posted: Wed Jul 11, 2007 8:59 pm Post subject: |
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marlasinger wrote: | from some random sites:
" One way to repair mRNA is to use ribozymes, RNA molecules that act as 'molecular scissors' by catalysing the cleavage of mRNA at sequence-specific sites."
"Targeting a mutation discovered in an affected dog, they engineered a ribozyme that splices off the mutation-containing 3? section of the mRNA and replaces it with the wild-type sequence. This technique is called ribozyme-mediated trans-splicing."
http://www.vetscite.org/publish/items/001060/index.html
knowing the function and theories of ribozymes will make filling in those blanks a LOT easier, guys. |
which is why i got most of it the first time through
we still need to knowww why it needs to be made complementary in order to: osadoiadoaihBLANK _________________ -Kelly |
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marlasinger Devoted Fan

Joined: 27 Feb 2007 Posts: 736 Location: The 8th Dimension
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Posted: Wed Jul 11, 2007 9:00 pm Post subject: |
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I guess what I'm trying to say is..
Instead of focusing on the words we DON'T have, let's work with the words we DO have. Words like...Intron. Exon. fructose-6-phosphate. Things we can research. If we can't get the sentence, we can get the relationship between ribozymes and these substances. okay? _________________ my love tastes a lot like tofu.
The Bree Solution.
http://one.revver.com/watch/330795
"Also... im in ur canons... supporting all ur marlas."
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SivartAlappes Lonely Fan

Joined: 02 Oct 2006 Posts: 209 Location: Virginia but moving to Cali soon
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Posted: Wed Jul 11, 2007 9:00 pm Post subject: |
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kellylen wrote: |
can it occlude its copy? or does that make no sense? |
In chemistry, occlude means to incorporate something by absorbtion... so yes, in this case (we're making a synthetic copy of the ribozyme) the original not only CAN occlude it's copy, it must. |
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Aponi Devoted Fan

Joined: 26 Apr 2007 Posts: 545 Location: New York
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Posted: Wed Jul 11, 2007 9:01 pm Post subject: |
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Pag? Sociat? Reference Points.
Just saying what the words look like. I think the last letter of the first one is a g.
And if I need to know about RNA i'm kinda screwed.  _________________ The names Julie.
Bree and Jonas Forever! <3
Grand Master of Praise of the SSJF but Shhh about it 'k? XD
My LG15 Vid |
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kellylen The Order of Denderah

Joined: 21 Nov 2006 Posts: 2823 Location: New Jersey
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Posted: Wed Jul 11, 2007 9:02 pm Post subject: |
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well whats his face has gotten most of the bottom things and they are right. i did some research and each time they posted something i was about to say that XD _________________ -Kelly |
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Raven_Night Casual Observer

Joined: 09 May 2007 Posts: 78 Location: In My Own World
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Posted: Wed Jul 11, 2007 9:02 pm Post subject: |
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______related - telomere synthesis
Quote: | Replication of linear chromosomes presents a special problem.
DNA polymerase can only synthesize a new strand of DNA as it moves along the template strand in the 3' –> 5' direction. This works fine for the 3' –> 5' strand of a chromosome as the DNA polymerase can move uninterruptedly from an origin of replication until it meets another bubble of replication or the end of the chromosome. However, synthesis using the 5' –> 3' strand as the template has to be discontinuous. When the replication fork opens sufficiently, DNA polymerase can begin to synthesize a section of complementary strand — called an Okazaki fragment — working in the opposite direction. Later, DNA ligase stitches the Okazaki fragments together. |
^^taken from a site about telomore
Last edited by Raven_Night on Wed Jul 11, 2007 9:04 pm; edited 1 time in total |
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kellylen The Order of Denderah

Joined: 21 Nov 2006 Posts: 2823 Location: New Jersey
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Posted: Wed Jul 11, 2007 9:03 pm Post subject: |
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SivartAlappes wrote: | kellylen wrote: |
can it occlude its copy? or does that make no sense? |
In chemistry, occlude means to incorporate something by absorbtion... so yes, in this case (we're making a synthetic copy of the ribozyme) the original not only CAN occlude it's copy, it must. |
then occlude the copy makes sense in order to block the other one to stop the whole thing ... thing _________________ -Kelly |
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simplykimmie13 Casual Observer

Joined: 01 Jul 2007 Posts: 72 Location: my room.
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Posted: Wed Jul 11, 2007 9:03 pm Post subject: |
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kellylen wrote: |
4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine
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i think its blend not block it. _________________ Soo the name is Kimmie and i basically rock socks. how bout you?
**Member of the Spencer Gilman fan club! woo!** |
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TOSG Devoted Fan

Joined: 14 Sep 2006 Posts: 651
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Posted: Wed Jul 11, 2007 9:06 pm Post subject: |
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I THINK that c) should be "Hammerhead related - telomere synthesis", but the lettering is damn near impossible to make out.
Also, it's "the gene," not "genome," for given fact 2). _________________ I'm canon; the Creators just don't know it yet. |
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ohpete Casual Observer

Joined: 11 Jul 2007 Posts: 40
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Posted: Wed Jul 11, 2007 9:06 pm Post subject: |
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*slams keyboard*
photoshop still wont open, and now im sitting here trying searches for anything i can think of, because google hasnt helped me to fill in the blanks any more at this point. [though i know a lot more about this stuff now.] |
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