Bree's injections

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Bree's injections became the subject of a great deal of debate since the video House Arrest, in which Bree is wearing a bandage because she had to receive an injection.

These are given to her by her father but he claims that he does not know what they contain. Fans have long speculated on the contents and purpose of these injections, but it still remains unclear.

Vitamin B-12

For a long time the leading theory was that the injections contained vitamin B-12 to stimulate blood production. If this were the case, Bree's participation in the ceremony would likely involve blood being drawn from her. Bree's preparation for the ceremony also requires her to take iron supplements, which also aid in the production of blood. In this sense, it's logical to think that the shots may contain vitamin B-12. However, Bree has described that these shots often leave her feeling unwell, which should not be the case if they are merely vitamin B12 supplements.

Cerebyx and Zosyn

Daniel found these in Bree's parents room.
Another picture of the pamphlets.

After Daniel posted the video Back At Bree's, fans began to analyse his clip for clues about Bree's ceremony. Two pamphlets caught viewers' eyes. They were on what seemed to be Bree's parents' bed. As Daniel was exploring, he panned his camera across the bed, producing only some blurry shots of the covers of these pamphlets. Fans claim to have identified that they are informative pamphlets for the drugs Cerebyx and Zosyn. Cerebyx is an anticonvulsive medication that has had some experimental testing as a pain medication. Zosyn is an antibiotic.

At the end of his video, Daniel said he had found something, but he does not reveal what it was. Many viewers thought he would eventually reveal these pamphlets. In his next video, Undisclosed Location, Daniel explains that what he found was a box with a lemniscate imprinted on it. Once he showed that it was the box that held significance to him, the pamphlets became of less importance. It is unlikely that he would have noticed the box and not the pamphlets, so it's likely that he saw them but did not think they were important. Bree's father is a doctor, and as such probably has many more marketing materials from drug companies strewn about the bedroom other than the ones seen in the video.

Other suggestions

Viewers have also suspected that the injections contain mood-altering drugs.